Oocytes

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Xenopus Oocytes

lnjection of Xenopus oocytes with poly(A)+ mRNA isolated from different plants (maize, cucumber, and squash) results in the appearance of a voltageand time'dependent, potassium-selective, outward current that is similar to the outwardrectifying potassium current recorded in many higher plant cells. Maize shoots were found to be especially enriched in mRNA encoding such activity. A cDNA library ...

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Xenopus oocytes

The induction of amphibian oocyte maturation with progesterone as well as the activation of sea urchin eggs at the time of fertilization result in increased protein synthesis. The increase in both cases involves the recruitment of maternal mRNA onto polysomes. Further, it has been reported that sea urchin eggs, like full-grown Xenopus oocytes, contain no spare translational capacity based on th...

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Actin in Xenopus oocytes

It has been found that a high-speed supernatant fraction from Xenopus oocytes extracted in the cold will form a clear, solid gel upon warming. Gel formation occurs within 60 min at 18 degrees-40 degrees C, and is, at least initially, temperature reversible. Gelation is strictly dependent upon the addition of sucrose to the extraction medium. When isolated in the presence of ATP, the gel consist...

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Lytechinus pictus oocytes

The annexins are a family of calcium-dependent phospholipidbinding proteins, of which there are known to be at least ten mammalian genes (Moss, 1992), with further unrelated annexins identified in Hydra vulgaris and Drosophila melanogaster (Schlaepfer et al., 1992a; Gerke, 1989). Despite a lack of persuasive functional data, the annexins have been proposed to have roles as phospholipase A2 inhi...

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Enucleated Oocytes and Zygotes

The present study was conducted to examine the development of nucLear transplant embryos produced by transplanting nucLei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, fourand eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without st...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2000

ISSN: 0268-1161,1460-2350

DOI: 10.1093/humrep/15.suppl_4.11